Development of a novel BIA enzyme calorimetric biosensor and detection system for hydrogen peroxide determination at hazardous level

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 會議論文 > Item 310904400/8762

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Title:	Development of a novel BIA enzyme calorimetric biosensor and detection system for hydrogen peroxide determination at hazardous level
Authors:	Wei, Hsien-Chin;Huang, Su-Hua;Lee, Yeun-Chung
Contributors:	Department of Biotechnology
Keywords:	Calorimetry;Enzymes;Functional polymers;Hydrogen;Hydrogen peroxide;Interfaces (computer);Oxidation;Personal computers;Pumps;Signal transduction;Temperature measurement;Thermal effects;Thermistors -Analog-to-digital conversions;Batch-injection-analysis (BIA);Biocalorimetry;Calorimetric biosensor;Calorimetric sensing;Computer programs;Correlation coefficients;Detection systems;High precision;Hydrogen peroxide concentrations;Hydrogen peroxide sensors;Linear ranges;Low costs;Medical thermistor;Polymeric reactions;Reaction cells;Reaction mediums;Serial bus;Single-chip microcontrollers;Temperature changes;Temperature profiles
Date:	2008-06
Issue Date:	2010-04-08 11:17:50 (UTC+0)
Publisher:	Asia University
Abstract:	This work presents optical enzyme detection system based on the CMOS array photo sensor and 1 × 3 polymeric enzyme biochip for detecting Escherichia coli in a one-step procedure. This assay, using 4-methylumbelliferyl-β-d-glucuronide (MUG) as a fluorogenic substrate, had a detection limit of 0.1 U/ml for β-glucuronidase (GUD), which was approximately equal to a cell concentration of 10<sup>6</sup> CFU/ml of E. coli. MUG was incorporated into lauryl tryptose broth at a final concentration of 100 μg/ml for immediate verification of the presence of E. coli in 1 × 3 polymeric enzyme biochip. The 40 strains of E. coli studied all produced GUD. Of another 36 strains of bacteria tested, one strain (Salmonella choleraesuis subsp. choleraesuis) yielded very small amounts of GUD after 24 h incubation. The optical enzyme detection system was sensitive and rapid. © 2008 Elsevier B.V. All rights reserved.
Relation:	American Society of Agricultural and Biological Engineers Annual International Meeting 2008
Appears in Collections:	[生物科技學系] 會議論文

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