ASIA unversity:Item 310904400/8762
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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/8762


    Title: Development of a novel BIA enzyme calorimetric biosensor and detection system for hydrogen peroxide determination at hazardous level
    Authors: Wei, Hsien-Chin;Huang, Su-Hua;Lee, Yeun-Chung
    Contributors: Department of Biotechnology
    Keywords: Calorimetry;Enzymes;Functional polymers;Hydrogen;Hydrogen peroxide;Interfaces (computer);Oxidation;Personal computers;Pumps;Signal transduction;Temperature measurement;Thermal effects;Thermistors -Analog-to-digital conversions;Batch-injection-analysis (BIA);Biocalorimetry;Calorimetric biosensor;Calorimetric sensing;Computer programs;Correlation coefficients;Detection systems;High precision;Hydrogen peroxide concentrations;Hydrogen peroxide sensors;Linear ranges;Low costs;Medical thermistor;Polymeric reactions;Reaction cells;Reaction mediums;Serial bus;Single-chip microcontrollers;Temperature changes;Temperature profiles
    Date: 2008-06
    Issue Date: 2010-04-08 11:17:50 (UTC+0)
    Publisher: Asia University
    Abstract: This work presents optical enzyme detection system based on the CMOS array photo sensor and 1 × 3 polymeric enzyme biochip for detecting Escherichia coli in a one-step procedure. This assay, using 4-methylumbelliferyl-β-d-glucuronide (MUG) as a fluorogenic substrate, had a detection limit of 0.1 U/ml for β-glucuronidase (GUD), which was approximately equal to a cell concentration of 10<sup>6</sup> CFU/ml of E. coli. MUG was incorporated into lauryl tryptose broth at a final concentration of 100 μg/ml for immediate verification of the presence of E. coli in 1 × 3 polymeric enzyme biochip. The 40 strains of E. coli studied all produced GUD. Of another 36 strains of bacteria tested, one strain (Salmonella choleraesuis subsp. choleraesuis) yielded very small amounts of GUD after 24 h incubation. The optical enzyme detection system was sensitive and rapid. © 2008 Elsevier B.V. All rights reserved.
    Relation: American Society of Agricultural and Biological Engineers Annual International Meeting 2008
    Appears in Collections:[Department of Biotechnology] Proceedings

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