Short-Term versus Long-Term Intermittent Hypobaric Hypoxia on Cardiac Fibrosis and Fas Death Receptor Dependent Apoptotic Pathway in Rat Hearts

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Title:	Short-Term versus Long-Term Intermittent Hypobaric Hypoxia on Cardiac Fibrosis and Fas Death Receptor Dependent Apoptotic Pathway in Rat Hearts
Authors:	Lin, YM;Lin, Yueh-Min;Huang, SK;Huang, Shu-Kuei;Wang, HF;Wang, Hsuch Fang;Chen, LM;Chen, Li-Mien;Tsai, FJ;Tsai, Fuu-Jen;Hsu, HH;Hsu, Hsi-Hsien;Kuo, CH;Kuo, Chia-Hua;Wang, PS;Wang, Paulus S.;Huang, CY;Huang, Chih-Yang;Lee, SD;Lee, Shin-Da
Contributors:	保健系
Keywords:	cardiac fibrosis;cell apoptosis;high altitude;Fas pathway;time course;OBESE ZUCKER RATS;INDUCED CARDIOMYOCYTE APOPTOSIS;DIFFERENT SIGNALING PATHWAYS;OPEN-ARTERY HYPOTHESIS;ISCHEMIA/REPERFUSION INJURY;ANGIOTENSIN-II;MMP-9 ACTIVITY;EXPRESSION;MYOCYTES;MECHANISMS
Date:	2008-10
Issue Date:	2010-03-26 02:46:39 (UTC+0)
Publisher:	Asia University
Abstract:	It is unknown if short-term and long-term intermittent hypobaric hypoxic challenges both exert pro-apoptotic effects on Fas death receptor-dependent apoptotic pathway in rat hearts. Seventy-two Sprague-Dawley rats were randomly assigned into two groups. First, short-term intermittent hypobaric hypoxia (STIHH)-normobaric normoxia (n = 12), hypobaric hypoxia (380 mmHg, 12% O-2, 8 hrs/day) for 1 day (n = 12), and for 4 days (n = 12) and second, long-term intermittent hypobaric hypoxia (LTIHH)-normobaric normoxia (n = 12), hypobaric hypoxia for 1 week (n = 12) and 2 weeks (n = 12). After STIHH or LTIHH challenge, Fas receptor related pathway and histopathological analysis in the excised left ventricle was determined by Western blotting, RT-PCR, Hematoxylin-eosin staining, Masson trichrome staining and TUNEL assay. Fas death receptor and TNF alpha were significantly decreased after STIHH whereas Fas receptor, TNF alpha, FAS-associated death domain (FADD), and caspase 8 were increased after LTIHH. In addition, cardiomyocyte disarray and fibrosis were observed in 1 week LTIHH. Cardiac hypertrophy and more severe disarray, fibrosis and cardiac apoptotic activities were observed in 2 week LTIHH. STIHH exerts anti-apoptotic effects on hearts such as downregulation of TNF alpha and Fas receptor whereas LTIHH exerts pro-apoptotic effects such as upregulation of TNF alpha and Fas-mediated apoptotic pathways and lead to cardiac fibrosis and apoptosis. Our findings imply that short-term versus long-term intermittent hypobaric hypoxia exerted protective versus deleterious effects on hearts.
Relation:	CHINESE JOURNAL OF PHYSIOLOGY, 51(5):308-316.
Appears in Collections:	[食品營養與保健生技學系] 期刊論文

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