A potential methodology for differentiation of ciguatgoxin-carrying species of moray eel

ASIA unversity > 醫學暨健康學院 > 食品營養與保健生技學系 > 期刊論文 > Item 310904400/8399

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Title:	A potential methodology for differentiation of ciguatgoxin-carrying species of moray eel
Authors:	Tsai, WL;Tsai, Wei Lung;Chen, HM;Chen, Hong-Ming;Hsieh, CH;Hsieh, Cheng-Hong;Lin, WF;Lin, Wen-Feng;Hwang, DF;Hwang, Deng-Fwu
Contributors:	保健系
Keywords:	Moray eel;Ciguatera;Food poisoning;PCR-RFLP;Cytochrome b gene;MITOCHONDRIAL-DNA SEQUENCES;CYTOCHROME-B GENE;PCR-RFLP ANALYSIS;PHYLOGENETIC-RELATIONSHIPS;CARIBBEAN CIGUATOXINS;CICHLID FISHES;IDENTIFICATION;CIGUATERA;PRODUCTS;FRAGMENT
Date:	2009-06
Issue Date:	2010-03-26 02:46:25 (UTC+0)
Publisher:	Asia University
Abstract:	A food poisoning incident due to ingestion of an unknown moray eel occurred in Taiwan. To identify the species of causative moray eel implicated in food poisoning. a 376-bp fragment sequence of cytochrome b gene was successfully obtained from four species of moray eel by using a pair of primers (L newest-1/H 15149). This fragment could be amplified using fish meat treated with different heating processes (100 degrees C for 30 min, 100 degrees C for 60 min, 121 degrees C for 15 min, and 121 degrees C for 30 min). After sequencing, it was found that no variation in sequence was detected among individuals within each species. The species of causative moray eel implicated in food poisoning was judged as Gymnothorax javanicus based on sequence analysis. In addition, using restriction enzyme analysis, including Taq 1 and Sau96 1, could distinguish these four species of moray eel and identify the fish species of poisoning sample as G. javanicus. The toxicity of viscera in 24 specimens of four moray eel species was not detected, but the food poisoning sample was found to be toxic. Overall, this study proved that DNA. sequence and restriction enzyme analyses are useful in identifying that the causative moray eel species was G. javanicus. (C) 2008 Elsevier Ltd. All rights reserved.
Relation:	FOOD CONTROL ,20(6):575-579.
Appears in Collections:	[食品營養與保健生技學系] 期刊論文

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