Aloe-emodin is an interferon-inducing agent with antiviral activity against Japanese encephalitis virus and enterovirus 71

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Title:	Aloe-emodin is an interferon-inducing agent with antiviral activity against Japanese encephalitis virus and enterovirus 71
Authors:	Lin, CW (Lin, Cheng-Wen);Wu, CF (Wu, Chia-Fang);Hsiao, NW (Hsiao, Nai-Wan);Chang, CY (Chang, Ching-Yao);Li, SW (Li, Shih-Wein);Wan, L (Wan, Lei);Lin, YJ (Lin, Ying-Ju);Lin, WY (Lin, Wei-Yong)
Contributors:	Department of Biotechnology
Keywords:	aloe-emodin;interferon signalling;Japanese encephalitis virus;enterovirus 71;NITRIC-OXIDE;IN-VITRO;INFECTION;ANTHRAQUINONES;DERIVATIVES;INHIBITION;ACTIVATION;MICE
Date:	2008-10
Issue Date:	2010-03-26 02:30:05 (UTC+0)
Publisher:	Asia University
Abstract:	In this study, aloe-emodin was identified as a potential interferon (IFN)-inducer by screening compounds from Chinese herbal medicine. Aloe-emodin showed low cytotoxicity to human HL-CZ promonocyte cells and TE-671 medulloblastoma cells and significantly activated interferon-stimulated response element (ISRE) and gamma-activated sequence (GAS)-driven cis-reporting systems. Moreover, aloe-emodin upregulated expression of IFN-stimulated genes such as dsRNA-activated protein kinase and 2', 5'-oligoisoadenylate synthase. Aloe-emodin resulted in significant activation of nitric oxide production. The antiviral activity of aloe-emodin against Japanese encephalitis virus (JEV) and enterovirus 71 (EV71) was evaluated using dose- and time-dependent plaque reduction assays in HL-CZ cells and TE-671 cells. The 50% inhibitory concentration (IC50) of aloe-emodin ranged from 0.50 mu g/mL to 1.51 mu g/mL for JEV and from 0.14 mu g/mL to 0.52 mu g/mL for EV71. Aloe-emodin showed clearly potent virus inhibitory abilities and achieved high therapeutic indices, in particular for HL-CZ cells. Therefore, the study demonstrated dose- and time-dependent actions of aloe-emodin on the inhibition of JEV and EV71 replication via IFN signalling responses. (C) 2008 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.
Relation:	INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 32 (4): 355-359
Appears in Collections:	[Department of Biotechnology] Journal Article

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