Regulation of the pehA gene encoding the major polygalacturonase of Xanthomonas campestris by CIp and RpfF

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/8349

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Title:	Regulation of the pehA gene encoding the major polygalacturonase of Xanthomonas campestris by CIp and RpfF
Authors:	Hsiao, YM (Hsiao, Yi-Min);Zheng, MH (Zheng, Mei-Huei);Hu, RM (Hu, Rouh-Mei);Yang, TC (Yang, Tsuey-Ching);Tseng, YH (Tseng, Yi-Hsiung)
Contributors:	Department of Biotechnology
Keywords:	CELL-CELL COMMUNICATION;PATHOGENICITY FACTOR PRODUCTION;GRAM-NEGATIVE BACTERIA;AMP RECEPTOR PROTEIN;PHAGE PHI-LF;PV CAMPESTRIS;ERWINIA-CHRYSANTHEMI;PATHOVAR CAMPESTRIS;XANTHAN GUM;TRANSCRIPTION FACTOR
Date:	2008-03
Issue Date:	2010-03-26 02:29:59 (UTC+0)
Publisher:	Asia University
Abstract:	Exopolysaccharide and several extracellular enzymes of Xanthomonas campestris pv. campestris (Xcc), the causative agent of black rot in crucifers, are virulence factors. In this study, sequence and mutational analysis has demonstrated that Xcc pehA encodes the major polygalacturonase, a member of family 28 of the glycosyl hydrolases. Using the 5' RACE (rapid amplification of cDNA ends) method, the pehA transcription initiation site was mapped at 102 nt downstream of a CIp (cAMP receptor protein-like protein)-binding site. Transcriptional fusion assays showed that pehA transcription is greatly induced by polygalacturonic acid, positively regulated by CIp and RpfF (an enoyl-CoA hydratase homologue which is required for the synthesis of cis-11-methyl-2-dodecenoic acid, a low-molecular-mass diffusible signal factor), subjected to catabolite repression, which is independent of CIp or RpfF, and repressed under conditions of oxygen limitation or nitrogen starvation. Our findings extend previous work on CIp and RpfF regulation to show that they both influence the expression of pehA in Xcc.
Relation:	MICROBIOLOGY-SGM, 154: 705-713 Part 3
Appears in Collections:	[生物科技學系] 期刊論文

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