Genetic analysis of an attenuated Papaya ringspot virus strain applied for cross protection.

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/8095

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://asiair.asia.edu.tw/ir/handle/310904400/8095

题名:	Genetic analysis of an attenuated Papaya ringspot virus strain applied for cross protection.
作者:	Chiang, C.-H.;Lee, C.-Y.;Wang, C.-H.;Jan, F.-J.;Lin, S.-S.;Chen, T.-C.;Raja, J. A. J.;Yeh S.-D
贡献者:	Department of Biotechnology
关键词:	Attenuated virus;Cross-protection;In vitro transcription;Recombinant virus
日期:	2007
上传时间:	2010-03-15 08:12:19 (UTC+0)
出版者:	Asia University
摘要:	Papaya ringspot virus (PRSV) HA 5-1, a nitrous acid-induced mild mutant of severe strain HA, widely applied for control of PRSV by cross-protection, was used to study the genetic basis of attenuation. Using infectious clones, a series of recombinants was generated between HA 5-1 and HA and their infectivity was analyzed on the systemic host papaya and the local lesion host Chenopodium quinoa. The recombinants that contained mutations in P1 and HC-Pro genes caused attenuated infection on papaya without conspicuous symptoms, similar to HA 5-1. The recombination and sequence analyses strongly implicated two amino acid changes in the C-terminal region of P1 and two in HC-Pro of HA 5-1 involved in the attenuated infection on papaya. The recombinants that infected C. quinoa plants without local lesions contained the same mutations in the C-terminal region of HC-Pro for attenuated infection on papaya. We conclude that both P1 and HC-Pro bear important pathogenicity determinants for the infection on the systemic host papaya and that the mutations in HC-Pro affecting pathogenicity on papaya are also responsible for the inability to induce hypersensitive reaction on C. quinoa.
關聯:	European Journal of Plant Pathology 118:333-348
显示于类别:	[生物科技學系] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
		0Kb	Unknown	762	检视/开启
310904400-8095.doc		37Kb	Microsoft Word	288	检视/开启

在ASIAIR中所有的数据项都受到原著作权保护.

数据加载中.....