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    题名: Identification of a novel septin 4 protein binding to human herpesvirus 8 kaposin A protein using a phage display cDNA library
    作者: Lin, C. W.;Tu, P. F.;Hsiao, N. W.;Chang, C. Y.;Wan, L.;Lin, Y. T.;Chang, H. W.
    贡献者: Department of Biotechnology
    关键词: Virus;Herpesviridae;Gammaherpesvirinae;Virology;Method;Microbiology;Complementary DNA;Phage display;Binding protein;Identification;Human herpesvirus 8
    日期: 2007
    上传时间: 2010-03-15 08:11:21 (UTC+0)
    出版者: Asia University
    摘要: Human herpesvirus 8 (HHV-8) is associated with the development of Kaposi's sarcoma and several other human malignancies. Kaposin A protein of HHV-8 has been demonstrated as inducing tumorigenic transformation, being responsible for nuclear receptor coactivators in the transforming activity. In this study, a kaposin A-interacting septin 4 variant that contained the unique GDR at the N-terminus and AAALE at the C-terminus was identified using affinity selection of a phage display library. Co-immunoprecipitation and confocal imaging revealed in vitro binding specificity and in vivo co-localization of HHV-8 kaposin A protein to the septin 4 variant. The kaposin A-interacting septin 4 variant induced cell rounding up, activated caspase-3, and up-regulated transcriptional factor NF-KB. By contrast, kaposin A protein showed an antagonistic effect on the biological functions of the septin 4 variant. Therefore, the interaction of kaposin A protein and the septin 4 variant was suggested as playing a possible role in the development of HHV-8-associated malignancies. This study provides insights into the mechanism of the kaposin A protein pathology, in which the interactions of kaposin A protein with cellular proteins might allow alteration of fundamental cellular processes.
    關聯: Journal of Virological Methods 143:65-72
    显示于类别:[生物科技學系] 期刊論文

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