Stabilization of calpain large subunits by overexpression of truncated calpain small subunit in L8 myoblasts.

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Title:	Stabilization of calpain large subunits by overexpression of truncated calpain small subunit in L8 myoblasts.
Authors:	Chung, T. C.;Liang, Y. C.;Yeh, J. Y.;Ou, B. R.
Contributors:	Department of Biotechnology
Keywords:	Truncated calpain small subunit;Calpain large subunit;Stability;L8 myoblast
Date:	2004
Issue Date:	2010-03-15 08:11:04 (UTC+0)
Publisher:	Asia University
Abstract:	The objectives were to investigate the function of the small subunit in the calpain system by expression of the autolytic form of this subunit in L8 myoblasts. Rat post-autolysis small subunit (21 kDa) cDNA expression plasmid was transfected into L8 myoblasts and selected by G418 containing medium. The concentrations of cytosolic -calpain in transfected cells, SS2 and SS3, were found to be 15.7 and 17.3% higher than that in L8Neo control cells, and the concentrations of cytosolic m-calpain in SS2 and SS3 cells were 23.3 and 16.6% higher than that in control cells (L8Neo). The half-life of -calpain in SS3 cells (36.5 h) was longer than that in L8Neo cells (32.4 h), while the half-life of m-calpain in SS3 cells (40.1 h) was longer than that in L8Neo cell (37.5 h). These results indicated that the expression of truncated small subunit increased the stability of - and m-calpain large subunits in cytosol.
Relation:	Tissue & Cell 36:181-187
Appears in Collections:	[Department of Biotechnology] Journal Article

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