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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/7900


    Title: 榕樹細菌性癌腫病菌Agrobacterium tumefaciens在台灣之發生
    Authors: 許秀惠;林俊義;陳福旗
    Contributors: Department of Biotechnology
    Keywords: 榕樹,癌腫病,癌腫病菌,鑑定
    Date: 1997
    Issue Date: 2010-03-15 08:09:34 (UTC+0)
    Publisher: Asia University
    Abstract: 於田尾地區田間及盆栽栽培之金門榕(Ficus microcarpa L. f. var. pusilifolia Liao cv. Kin-men),中葉榕(Ficus microcarpa L. f. cv. Middle-leaf)及屏東地區之小葉榕(Ficus microcarpa L. f. var. pusilifolia Liao)植株上發現腫瘤病,腫瘤大小可達10-20餘公分,主要發生於根冠、樹幹或枝條之修剪處或其它傷口處。由腫瘤處分離所得之細菌在PDA培養基上形成黏濕,圓形具亮光,不透明的白色菌落,為革蘭氏陰性,好氣性,具有1-6根週生鞭毛的桿狀細菌。該細菌以Biolog GN Microplate鑑定結果與Agrobacterium tumefaciens相近,再依選擇性培養基及各種生理生化試驗結果屬於biovar 1。該細菌接種於胡蘿蔔肉質根切塊上,經4-5天後於形成層處形成淡綠色癒合組織狀之腫大,但未出現根狀增生;以針刺及剪枝條的方法接種於盆栽金門榕及中葉榕之枝條上及農友301番茄植株之莖或葉柄處,榕樹於接種後約三星期於接種處出現淡黃色小腫瘤,顏色隨著腫瘤增大而加深,而番茄植株則於接種後7-10天於接種處形成淡白綠色之腫瘤,之後瘤漸增大且顏色轉為淡黃色,整個瘤約可大到1公分,且在近感染處同側的葉片呈現失水狀,該葉片最後萎凋。根據上述試驗結果顯示造成榕樹癌腫病之病原菌為Agrobacterium tumefaciens biovar 1。
    Relation: 植保會刊 39:195-205
    Appears in Collections:[生物科技學系] 期刊論文

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