Identification of tetrodotoxin and fish species in dried dressed fish fillet implicated in food poisoning

ASIA unversity > 醫學暨健康學院 > 食品營養與保健生技學系 > 期刊論文 > Item 310904400/7798

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题名:	Identification of tetrodotoxin and fish species in dried dressed fish fillet implicated in food poisoning
作者:	Hwang, D. F.;Y. W. Hsieh;Y. C. Shiu;S. K. Chen;C. A. Cheng
关键词:	Free Content;New Content;Subscribed Content;Free Trial Content
日期:	2002
上传时间:	2010-02-26
出版者:	Asia University
摘要:	There were five victims of neurotoxic food poisoning from a dried dressed fish fillet in Changhua County, Taiwan, in February 2000. The toxicity of the dried dressed fish fillets was 243 mouse units per g according to a tetrodotoxin bioassay. The partially purified toxin was identified as tetrodotoxin and anhydrotetrodotoxin. The sequence of the 376-nucleotide region in the cytochrome b gene of the mitochondrial DNA exhibited the same genotype as that of the toxic puffer fish Lagocephalus lunaris. The same single restriction site for HinfI was found in the polymerase chain reaction (PCR) products from the dried dressed fish fillet and the muscle of L. lunaris, yielding two DNA fragments of 170 and 206 bp. However, no restriction site for HinfI was found in the PCR products from other toxic puffer fishes, including Takifugu niphobles, Takifugu oblongus, and Takifugu rubripes. Therefore, the species of the dried dressed fish fillet was identified as L. lunaris and its causative agent was identified as tetrodotoxin.
關聯:	Journal of Food Protection 65:389-392
显示于类别:	[食品營養與保健生技學系] 期刊論文

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