Identification of tetrodotoxin and fish species in dried dressed fish fillet implicated in food poisoning

ASIAIR > College of Medical and Health Science > Department of Food Nutrition and Healthy Biotechnology > Journal Article > Item 310904400/7798

Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/7798

Title:	Identification of tetrodotoxin and fish species in dried dressed fish fillet implicated in food poisoning
Authors:	Hwang, D. F.;Y. W. Hsieh;Y. C. Shiu;S. K. Chen;C. A. Cheng
Keywords:	Free Content;New Content;Subscribed Content;Free Trial Content
Date:	2002
Issue Date:	2010-02-26
Publisher:	Asia University
Abstract:	There were five victims of neurotoxic food poisoning from a dried dressed fish fillet in Changhua County, Taiwan, in February 2000. The toxicity of the dried dressed fish fillets was 243 mouse units per g according to a tetrodotoxin bioassay. The partially purified toxin was identified as tetrodotoxin and anhydrotetrodotoxin. The sequence of the 376-nucleotide region in the cytochrome b gene of the mitochondrial DNA exhibited the same genotype as that of the toxic puffer fish Lagocephalus lunaris. The same single restriction site for HinfI was found in the polymerase chain reaction (PCR) products from the dried dressed fish fillet and the muscle of L. lunaris, yielding two DNA fragments of 170 and 206 bp. However, no restriction site for HinfI was found in the PCR products from other toxic puffer fishes, including Takifugu niphobles, Takifugu oblongus, and Takifugu rubripes. Therefore, the species of the dried dressed fish fillet was identified as L. lunaris and its causative agent was identified as tetrodotoxin.
Relation:	Journal of Food Protection 65:389-392
Appears in Collections:	[Department of Food Nutrition and Healthy Biotechnology] Journal Article

Files in This Item:

File	Description	Size	Format
		0Kb	Unknown	554	View/Open
310904400-7798 .doc		31Kb	Microsoft Word	306	View/Open