Immunochemistry of human plasma high density lipoproteins. Radioimmunoassay of apolipoprotein A-II

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Title:	Immunochemistry of human plasma high density lipoproteins. Radioimmunoassay of apolipoprotein A-II
Authors:	Mao SJT;Gotto AM;Jackson RL.
Contributors:	Department of Biotechnology
Date:	1975
Issue Date:	2009-11-26 01:43:44 (UTC+0)
Publisher:	Asia University
Abstract:	Apolipoprotein A-I1 (ApoA-11) is a major apoprotein of human plasma high density lipoproteins (HDL). The apoprotein has two identical chains of known amino acid sequence; the chains are linked by a single disulfide bond. In the present study, we have developed a specific radioimmunoassay for apoA-I1 that provides a convenient and reproducible method for measuring 10-100 ng of apoprotein. The assay was based on the competition of apoprotein with isotopically labeled [1251]apoA-IID. ioxane (52%) was used to separate antibody bound [1251]apoA-II from the free apoprotein. The assay has enabled us to begin studies directed toward mapping-out of the antigenic reactive regions of apoA-11. It has also allowed us to determine the interrelationship between the lipid-binding sites and the antigenic sites of the molecule. The antigenic reactivity of apoA-I1 was approximately the same in'HDL and phospholipid- apoA-I1 complexes as that of the free apoprotein. However, succinylation of apoA-I1 was associated with a marked decrease in antigenic reactivity.
Relation:	Biochemistry 14(18):4127–4131
Appears in Collections:	[Department of Biotechnology] Journal Article

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