Preparation of antibodies to a synthetic C terminus of hirudin and identification of an antigenic site

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題名:	Preparation of antibodies to a synthetic C terminus of hirudin and identification of an antigenic site
作者:	Mao SJT;Yates MT;Owen TJ;Krstenansky JL.
貢獻者:	Department of Biotechnology
日期:	1989-06
上傳時間:	2009-11-26 01:43:28 (UTC+0)
出版者:	Asia University
摘要:	Hirudin is a 65 amino acid anticoagulant peptide produced in the leech. The single polypeptide is cross-linked by three disulfide linkages in the NH2 terminal half of the molecule. A peptide corresponding to the COOH terminus (residues 45-65) was synthesized utilizing lysine 47 as a specific residue to conjugate to thyroglobulin as a carrier for raising antibodies in mice. Using an enzyme-linked immunosorbent assay (ELISA) technique, it was found that the major antigenic domain(s) was located between residues 52-65. The COOH terminal residues Ile-59, Tyr-63, and Leu-64 are crucial for maintaining the antigenic structure. The NH2 terminal region (residues 45-52) that is proximal to the carrier protein, however, was not immunoreactive. A possible mechanism by which antibodies recognize the COOH terminal region of the synthetic peptide and the strategy for raising such antibodies are discussed.
關聯:	Journal of Immunological Methods 120(1):45-50
顯示於類別:	[生物科技學系] 期刊論文

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