This research proposes to study the physical,chemical and genetic factors that could affects the grape (Vitis vinifera) anthocyanin biosynthesis pathway by integrating various kinds of molecular biology database information and through literature reviewing .Furthermore ,the kinetic parameter and upstream regulatory proteins of the anthocyanin are predicted based on the sequence homology principle. The grape anthocyanin biosynthesis pathway is retrieved from the KEGG database .Other utilized databases such as NCBI,BRENDA and SABIO-RK are useful for the collection of kinetic parameters. Several physical and chemical factors that could possibly enhanced the anthocyanin production are identified .It is also found that the Myb and Myc protein families could be the upstream regulating proteins for certain enzymes involved in the pathways . These results could be a useful piece of information for the grape anthocyanin biosynthesis pathway further study.