The drug stability include half life and ADME are the important factors that influence the drug efficacy. However, the resource of a number of drugs are come from proteins or peptides, which can be modified and reconstituted for every purpose. How to increase protein drug stability is the main challenge for pharmaceutical study. There are many ways to solve the problem. For example, we can enhance ionic pair, hydrophobic interaction, and hydrogen bond etc. Our tool provides prediction and modification for protein stability by calculate Temperature Index (TI) and Instability Index (II). The Temperature Index (TI) was applied to determine the Tm (Melting Temperature) directly from protein sequences. PDST(Protein Drug Stability Tuner) is a novel web online tool to provide analyzing protein drug stability and suggesting the mutation by inputting primary sequences of proteins. PDST is a freely available at http://biotuner.bio.ncue.edu.tw/pdst.htm We integrate PDB and Drug Bank, these two databases calculate the TI and II of these proteins, test and verify the result of analyzing. In according to the result, we build up a new database to provide the query of stability of protein drug.
