Survey of the Occurrence of Aristolochic Acid in Fangchi obtained from Mainland China by HPLC and The classification of the toxicity of traditional Chinese medicines

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题名:	Survey of the Occurrence of Aristolochic Acid in Fangchi obtained from Mainland China by HPLC and The classification of the toxicity of traditional Chinese medicines
作者:	Shang Hang YU
贡献者:	Department of Biotechnology
关键词:	Aristolochic Acid;HPLC;traditional Chinese medicines
日期:	2009
上传时间:	2009-11-06 06:11:26 (UTC+0)
出版者:	Asia University
摘要:	The purpose of this study was to investigate the present situation of the presence of Aristolochic Acid (AA) in Fangchi or Fangji crude drugs in China after issue of a warning on AA in November 1999. All Fangchi materials were purchased from the pharmacies of Traditional Chinese Medicines in Mainland China in 2006, and a total of twelve samples were collected. All samples arrived at our laboratory frozen and were stored at ≦ 4℃ until analysed. All extracts of samples were analysed on the same day as they were prepared. The samples were analyzed by HPLC on a Thermo Electron Corporation C12 column and detected at 254 nm with acetonitrile water and 2% (v/v) acetic acid (45:55, v/v) as the mobile phase at a flow rate of 0.8 ml/min. 34 samples of commericial fangchi were analyzed and the results showed that 6 samples contained aristolochic acid I and aristolochic acid II . The content of aristolochic acid I and aristolochic acid II in sample .
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