This study fully exploited the advantages of the similarities between chitosan and nitrocellulose (NC), their non-toxicity, superior germproof effects, and the characteristic of restraining fungal growth, to prevent NC’s biodegradation. In a comparison between NC’s Ea, the differences among them were dealt with or not with germproof chitosan by differential scanning calorimetry (DSC). We also observed specific functional groups with Fourier transform infrared (FT-IR) spectrometer to characterize the functional group transformation of NC under various thermal conditions.