Danthron induced apoptosis through mitochondria- and caspase-3-dependent pathways in human brain glioblastoma multiforms GBM 8401 cells

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/16480

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://asiair.asia.edu.tw/ir/handle/310904400/16480

题名:	Danthron induced apoptosis through mitochondria- and caspase-3-dependent pathways in human brain glioblastoma multiforms GBM 8401 cells
作者:	呂旭峰;Lu, Hsu-Feng;Wang, Hai-Lung;Chuang, Ying-Ying;Tang, Yih-Jing;楊家欣;Yang, Jai-Sing;Ma, Yi-Shih;江若華;Chiang, Jo-Hua;呂啟誠;Lu, Chi-Cheng;楊鈞隆;Yang, Jiun-Long;賴東淵;Lai, Tung-Yuan;Wu, Chih-Chung;鍾景光;Chung, Jing-Gung
贡献者:	生物科技學系
关键词:	Danthron;Apoptosis;Mitochondria;Caspase-3;GBM 8401 cells
日期:	2010-09
上传时间:	2012-11-23 09:13:39 (UTC+0)
摘要:	Danthron (1,8-dihydroxyanthraquinone), is one of component from Rheum palmatum L. (Polygonaceae), has been shown several biological activities but did not show to induce apoptosis in human brain tumor cells. The aim of this study is to investigate the mechanisms by danthron for the induction of apoptotic potential on human brain glioblastoma multiforms GBM 8401 cell line. Danthron showed a marked concentration- and time-dependent inhibition of GBM 8401 cell viability and induced apoptosis in a dose-and time-dependent manner. There was an attenuation of mitochondrial membrane potential (DeltaPsi(m)) with the alterations of Bcl-2/Bax protein ratio in GBM 8401 cells, indicating the participation of a mitochondria-related mechanism. Pretreatment of a caspase-8 inhibitor (Z-IETD-FMK), caspase-9 inhibitor (Z-LEHD-FMK) and caspase-3 inhibitor (Z-DEVE-FMK) significantly increased the viable of GBM 8401 cells implied that the participations of caspases. Western blotting analysis also showed the activation of initiator caspase-8 and caspase-9, and executor caspase-3 in GBM 8401 cells. Meanwhile, danthron also promoted the generation of reactive oxygen species (ROS) and cytosolic Ca(2+) in GBM 8401 cells. Taken together, our data showed that danthron induced apoptosis in GBM 8401 cells through mitochondria-related and caspase-related pathways, and it may be further evaluated as a chemotherapeutic agent for human brain cancer.
關聯:	NEUROCHEMICAL RESEARCH, 35(3):390-398.
显示于类别:	[生物科技學系] 期刊論文

文件中的档案:

没有与此文件相关的档案.

在ASIAIR中所有的数据项都受到原著作权保护.

数据加载中.....