Danthron induced apoptosis through mitochondria- and caspase-3-dependent pathways in human brain glioblastoma multiforms GBM 8401 cells

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Title:	Danthron induced apoptosis through mitochondria- and caspase-3-dependent pathways in human brain glioblastoma multiforms GBM 8401 cells
Authors:	呂旭峰;Lu, Hsu-Feng;Wang, Hai-Lung;Chuang, Ying-Ying;Tang, Yih-Jing;楊家欣;Yang, Jai-Sing;Ma, Yi-Shih;江若華;Chiang, Jo-Hua;呂啟誠;Lu, Chi-Cheng;楊鈞隆;Yang, Jiun-Long;賴東淵;Lai, Tung-Yuan;Wu, Chih-Chung;鍾景光;Chung, Jing-Gung
Contributors:	生物科技學系
Keywords:	Danthron;Apoptosis;Mitochondria;Caspase-3;GBM 8401 cells
Date:	2010-09
Issue Date:	2012-11-23 09:13:39 (UTC+0)
Abstract:	Danthron (1,8-dihydroxyanthraquinone), is one of component from Rheum palmatum L. (Polygonaceae), has been shown several biological activities but did not show to induce apoptosis in human brain tumor cells. The aim of this study is to investigate the mechanisms by danthron for the induction of apoptotic potential on human brain glioblastoma multiforms GBM 8401 cell line. Danthron showed a marked concentration- and time-dependent inhibition of GBM 8401 cell viability and induced apoptosis in a dose-and time-dependent manner. There was an attenuation of mitochondrial membrane potential (DeltaPsi(m)) with the alterations of Bcl-2/Bax protein ratio in GBM 8401 cells, indicating the participation of a mitochondria-related mechanism. Pretreatment of a caspase-8 inhibitor (Z-IETD-FMK), caspase-9 inhibitor (Z-LEHD-FMK) and caspase-3 inhibitor (Z-DEVE-FMK) significantly increased the viable of GBM 8401 cells implied that the participations of caspases. Western blotting analysis also showed the activation of initiator caspase-8 and caspase-9, and executor caspase-3 in GBM 8401 cells. Meanwhile, danthron also promoted the generation of reactive oxygen species (ROS) and cytosolic Ca(2+) in GBM 8401 cells. Taken together, our data showed that danthron induced apoptosis in GBM 8401 cells through mitochondria-related and caspase-related pathways, and it may be further evaluated as a chemotherapeutic agent for human brain cancer.
Relation:	NEUROCHEMICAL RESEARCH, 35(3):390-398.
Appears in Collections:	[Department of Biotechnology] Journal Article

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