Taiwanin E Induces Cell Cycle Arrest and Apoptosis in Arecoline/4-NQO-Induced Oral Cancer Cells Through Modulation of the ERK Signaling Pathway

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Title:	Taiwanin E Induces Cell Cycle Arrest and Apoptosis in Arecoline/4-NQO-Induced Oral Cancer Cells Through Modulation of the ERK Signaling Pathway
Authors:	王士豪;Wang, Shih Hao;Wu, Hsi-Chin;Wu, Hsi-Chin;Farheen, Khan;Badrealam, Khan Farheen;郭悅雄;Kuo, Yueh-Hsiung;Cha, Yun-Peng;Chao, Yun-Peng;Hs, Hsi-Hsien;Hsu, Hsi-Hsien;Bau, Da-Tian;Bau, Da-Tian;Padma, Vijaya;Viswanadha, Vijaya Padma;陳宜惠;CHEN, YI-HUI;Lio, Pei-Jei;Lio, Pei-Jei;黃志揚;Huang, Chih-Yang
Contributors:	醫學檢驗暨生物技術學系
Keywords:	Taiwanin E;apoptosis;cell cycle arrest;oral cancer;therapeutics.
Date:	2019-12
Issue Date:	2020-09-04 05:58:05 (UTC+0)
Publisher:	亞洲大學
Abstract:	Taiwanin E is a bioactive compound extracted from Taiwania cryptomerioides Hayata. In this research endeavor, we studied the anti-cancer effect of Taiwanin E against arecoline and 4-nitroquinoline-1-oxide-induced oral squamous cancer cells (OSCC), and elucidated the underlying intricacies. OSCC were treated with Taiwanin E and analyzed through MTT assay, Flow cytometry, TUNEL assay, and Western blotting for their efficacy against OSCC. Interestingly, it was found that Taiwanin E significantly attenuated the cell viability of oral cancer cells (T28); however, no significant cytotoxic effects were found for normal oral cells (N28). Further, Flow cytometry analysis showed that Taiwanin E induced G1cell cycle arrest in T28 oral cancer cells and Western blot analysis suggested that Taiwanin E considerably downregulated cell cycle regulatory proteins and activated p53, p21, and p27 proteins. Further, TUNEL and Western blot studies instigated that it induced cellular apoptosis and attenuated the p-PI3K/p-Akt survival mechanism in T28 oral cancer cells seemingly through modulation of the ERK signaling cascade. Collectively, the present study highlights the prospective therapeutic efficacy of Taiwanin E against arecoline and 4-nitroquinoline-1-oxide-induced oral cancer.
Relation:	Frontiers in Oncology
Appears in Collections:	[Department of Medical Laboratory Science and Biotechnology] Journal Article

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