AC-10 is a well-known traditional Chinese medicine in Taiwan. AC-10
possesses many bioactivities including anticancer activity. AC-10 showed
anti-breast cancer effect induced breast cancer cell apoptosis, however, the
metastasis was not clearly studied. TGF-β is a pleiotropic, anti-inflammatory
cytokine and a pivotal regulator of a wide range of cellular processes, including
cell cycle, proliferation, differentiation, epithelial-to-mesenchymal transition
(EMT) and metastasis. In cancer cells, EMT confers tumor cells with a distinct
advantage for metastatic dissemination. In this study, MDA-MB-231 cells were
treated with AC-10 (0-60 μg/mL) for 24 h. The protein levels of E-cadherin in
whole cells were determined by Western blotting. AC-10 up-regulates
E-cadherin expression. Results showed that AC-10 inhibited EMT via
modulation of Wnt/β-catenin and inhibition of Smad2/3 signaling pathways on
triple-negative breast cancer (MDA-MB-231) cells in vitro and in vivo.
