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http://asiair.asia.edu.tw/ir/handle/310904400/101591
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| Title: | Cellular thermotolerance is inheritable from Holstein cattle cloned with ooplasts of Taiwan native yellow cattle |
| Authors: | /;Piyawit, Kesorn;Lee, Jai-Wei;Lee, Jai-Wei;Wu, Hung-Yi;Wu, Hung-Yi;朱志成;Ju, Jyh-Cherng;*;Peng, Shao-Yu;Peng, Shao-Yu;Shyh-Shyan, L;Liu, Shyh-Shyan;Wu, His-Hsun;Wu, His-Hsun;Perng-Chih, S;Shen, Perng-Chih;* |
| Contributors: | 生物資訊與醫學工程學系 |
| Date: | 2016 |
| Issue Date: | 2016-11-08 02:12:53 (UTC+0) |
| Abstract: | We have previously demonstrated that the somatic cells from cattle cloned with Holstein (H) donor cells and Taiwan native yellow cattle (Y) ooplasm (Yo-Hd) had better thermotolerance than those from cattle cloned with both Holstein donor cells and ooplasm (Ho-Hd). The present study aimed to investigate whether the cellular thermotolerance of these cloned cattle is transmissible to their offspring (Ho-Hd-F1 and Yo-Hd-F1). Thermotolerance of ear fibroblasts derived from these cloned cattle and their offspring were analyzed. Polymorphisms in mitochondrial DNA (mtDNA) D-loop of ear fibroblasts derived from Yo-Hd and Yo-Hd-F1 indicated that the cytoplasm is originated from Bos indicus (Y). After heat shock, the apoptotic rates, B-cell lymphoma 2-associated X protein/B-cell lymphoma 2 ratios, and relative expression levels of cysteine-aspartic proteases (caspases)-3, -8, and -9 of ear fibroblasts with Y-originated cytoplasm (including Y, Yo-Hd, and Yo-Hd-F1) were lower (P < 0.05) than those of ear fibroblasts with H-originated cytoplasm (including H, Ho-Hd, and Ho-Hd-F1). In contrast, the relative level of HSP-70 was higher (P < 0.05) in ear fibroblasts with Y-originated cytoplasm than that of with H-originated cytoplasm. Based on our results, thermotolerance of ear fibroblasts derived from Yo-Hd and Yo-Hd-F1 cattle is better and can be transmitted, at least at the cellular level, to their offspring. |
| Relation: | THERIOGENOLOGY |
| Appears in Collections: | [生物資訊與醫學工程學系 ] 期刊論文
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