Cellular thermotolerance is inheritable from Holstein cattle cloned with ooplasts of Taiwan native yellow cattle

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题名:	Cellular thermotolerance is inheritable from Holstein cattle cloned with ooplasts of Taiwan native yellow cattle
作者:	/;Piyawit, Kesorn;Lee, Jai-Wei;Lee, Jai-Wei;Wu, Hung-Yi;Wu, Hung-Yi;朱志成;Ju, Jyh-Cherng;;Peng, Shao-Yu;Peng, Shao-Yu;Shyh-Shyan, L;Liu, Shyh-Shyan;Wu, His-Hsun;Wu, His-Hsun;Perng-Chih, S;Shen, Perng-Chih;
贡献者:	生物資訊與醫學工程學系
日期:	2016
上传时间:	2016-11-08 02:12:53 (UTC+0)
摘要:	We have previously demonstrated that the somatic cells from cattle cloned with Holstein (H) donor cells and Taiwan native yellow cattle (Y) ooplasm (Yo-Hd) had better thermotolerance than those from cattle cloned with both Holstein donor cells and ooplasm (Ho-Hd). The present study aimed to investigate whether the cellular thermotolerance of these cloned cattle is transmissible to their offspring (Ho-Hd-F1 and Yo-Hd-F1). Thermotolerance of ear fibroblasts derived from these cloned cattle and their offspring were analyzed. Polymorphisms in mitochondrial DNA (mtDNA) D-loop of ear fibroblasts derived from Yo-Hd and Yo-Hd-F1 indicated that the cytoplasm is originated from Bos indicus (Y). After heat shock, the apoptotic rates, B-cell lymphoma 2-associated X protein/B-cell lymphoma 2 ratios, and relative expression levels of cysteine-aspartic proteases (caspases)-3, -8, and -9 of ear fibroblasts with Y-originated cytoplasm (including Y, Yo-Hd, and Yo-Hd-F1) were lower (P < 0.05) than those of ear fibroblasts with H-originated cytoplasm (including H, Ho-Hd, and Ho-Hd-F1). In contrast, the relative level of HSP-70 was higher (P < 0.05) in ear fibroblasts with Y-originated cytoplasm than that of with H-originated cytoplasm. Based on our results, thermotolerance of ear fibroblasts derived from Yo-Hd and Yo-Hd-F1 cattle is better and can be transmitted, at least at the cellular level, to their offspring.
關聯:	THERIOGENOLOGY
显示于类别:	[生物資訊與醫學工程學系 ] 期刊論文

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