動態鎂離子與能量代謝物質分析在運動研究中之應用

ASIA unversity > 醫學暨健康學院 > 心理學系 > 科技部研究計畫 > Item 310904400/9701

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题名:	動態鎂離子與能量代謝物質分析在運動研究中之應用
作者:	鄭秀敏
贡献者:	人文學院心理學系
关键词:	自動採血;運動;能量代謝物質;鎂;微透析;Auto-blood sampling;Exercise;Energy metabolites;Magnesium;Microdialysis
日期:	2009
上传时间:	2010-05-19 05:42:57 (UTC+0)
摘要:	鎂廣泛存在生物體中，且為細胞中含量第二多的離子。鎂被認為在神經肌肉功能及能量代謝上扮演十分重要的角色。研究指出，長時間運動或極大運動量皆會造成低血鎂現象，然短時間運動或高強度運動則會造成高血鎂症。運動如何調控鎂離子的變化，仍舊是一個具有爭議性的問題。本研究計畫目標在發展自動採血系統與微透析分析技術以同時監測運動時動物血中與腦部之鎂離子、葡萄糖、乳酸以及丙酮酸濃度之動態變化。依實驗設計，雄性大鼠進行15分鐘游泳運動前預先給於硫酸鎂(90mg/kg，腹腔注射)或生理食鹽水，藉由自動採血系統收集靜脈血液及腦部微透析技術，利用微透析分析儀器(CMA 600)及原子吸收光譜儀同時監測分析血液與透析液之葡萄糖、乳酸、丙酮酸以及鎂離子濃度變化。本研究計劃結果可提供運動時鎂離子及能量代謝物質之動態變化之資訊，並可應用於研究不同運動型態之中樞與週邊之鎂離子與能量代謝物質的同步動態監測，增進了解運動、鎂離子與能量代謝物質的相互關係。 Magnesium is the second most abundant intracellular cation and presents in all living organisms. Magnesium is also regarded as an important element for neuromuscular functions and the efficiency of energy metabolism. Some studies have reported that prolonged and submaximal exercise induces hypomagnesaemia, whereas short-term, high-intensity exercise induces hypermagnesaemia. Whether exercise results in increased or decreased magnesium concentrations remains controversial. The aim of proposal is to develop auto-blood sampling method and microdialysis technique to detect the dynamic changes of glucose, lactate, pyruvate and magnesium levels of blood and brain in conscious and freely moving animals during exercise. Sprague-Dawley rats are pretreated with normal saline (control group) and magnesium sulfate (90 mg·kg-1, intraperitoneal injection) before a 15 min forced swimming. The dialysate and whole blood samples are continuously and simultaneously collected by the both microdialysis system and automated blood sampling system (automated blood sampler, Instech ABS). The dynamic profiles of glucose, lactate, pyruvate and magnesium before, during and after swimming are determined by microdialysis analyzer (CMA/600) and flame atomic absorption spectrometer (Perkin-Elmer Model 5100 FAAS). These data will provide dynamic information of central and peripheral magnesium as well as energy metabolites during exercise performance; moreover, the relationship of exercise, magnesium and energy metabolites levels will be further studied.
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