利用水稻T-DNA插入突變系進行發育與抗逆境功能性基因體研究－水稻T-DNA insertion特定突變品系種子之繁殖與特定基因大量篩檢流程之建立

ASIAIR > College of Medical and Health Science > Department of Biotechnology > Ministry of Science and Technology Research Project > Item 310904400/9450

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Title:	利用水稻T-DNA插入突變系進行發育與抗逆境功能性基因體研究－水稻T-DNA insertion特定突變品系種子之繁殖與特定基因大量篩檢流程之建立
Authors:	范宗宸
Contributors:	健康學院生物科技學系
Keywords:	T-DNA, 水稻側翼序列水稻功能性基因研究
Date:	2007
Issue Date:	2010-05-07 06:56:21 (UTC+0)
Abstract:	本計劃區分為兩個目標，第一為利用組織培養方法進行水稻種子胚拯救，目的係因為具有重大基因突變之下種子一般田間種植時受環境衝擊及植株間互相競爭，及所謂基因shift 現象，因此而被淘汰，而使珍貴突變基因在田間消失。利用胚拯救法及在隔離檢疫溫室中種植可排除以上之現象使T1 順利生長成T2 種子，供研究人員使用，且這些材料可以篩選到更多重要的突變種原。第二目標為配合整合型計劃中進行各項性狀之篩檢，如GA、ABA 抗逆境、鹽分、水分、溫度逆境、榖粒發育基因、病蟲害等基因篩檢時所發現之表現型具變異之株型，提供T-DNA 突變兩翼之DNA 序列，由於水稻基因組解序已接近完成,此結果可提供研究人員正向或逆向基因學之研究,進而達成功能性基因及基因之功能等之研究由於國家作物種原中心已保存60000 份T1 之T-DNA 突變水稻可供研究人員利用本計劃所獲得之兩翼序列將上傳至TRIM 資料庫,研究人員可進行進一步確認表現型與基因序列之關係,最終之目的為達成發現珍貴重要功能之基因序列，以為序列智慧財產權之申請。 There are two major targets for this project. The first purpose is using tissue culture to rescue those T-DNA rice which are poor grow in the field or had a bed germination rate, and can not harvest enough seeds for further research. Normally, that T-DNA rice which carries some important gene were incompatible to the wild type and will screen out in the filed, which call the gene drift. But this result is no our willing, because those important mutant genes will loss in this procedure. Using embryo rescue and nursing careful growth will prevent the problem mention above. The second propose for this project is to sequence the flanking sequence near by the T-DNA tagging. The results will complment those reseaschs such as screen for salt, drought, temperature, or GA, ABA regulation disorder or grain developing regulation and pest resistant phynotypes. Owing to the rice genome sequence is going to be completed, it creates an opportune for the forward or reverse genetic can be farther research by those researchers. And the goals of the rice functional genomic will be reach. There are more then 60,000 rice T-DNA T2 line had been generate and persevered in National Plant Genetic Resources Center, which are very important resources for rice functional genetic research used. After extract and purified the DNA from those rice T-DNA T1 lines, The flanking sequences generated from this research will be update in TRIM database. In this way the function respect to the sequence will be find out. Which not only can enhance the research result of our rice functional research, but also can discover the pressure sequence relate to the important function. Some results maybe can applied for intellectual property right application.
Appears in Collections:	[Department of Biotechnology] Ministry of Science and Technology Research Project

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