PAX3 Loads onto Pericentromeric Heterochromatin During S Phase Through PARP1

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/86926

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Title:	PAX3 Loads onto Pericentromeric Heterochromatin During S Phase Through PARP1
Authors:	吳祖方;TSU-FANG, WU;姚雅莉;Yao, Ya-Li;賴羿如;LAI, I-LU;李宗翰;LEE, TSUNG-HAN;UNDE, D.ALAN;UNDERHILL, D.ALAN;楊文明;YANG, WEN-MING
Contributors:	生物科技學系
Keywords:	PARP1;PAX3;S phase;pericentromeric heterochromatin
Date:	2014-09
Issue Date:	2014-11-07 06:51:51 (UTC+0)
Abstract:	BACKGROUND: Proper re-establishment of heterochromatin after each round of DNA replication is critical to the preservation of cell identity. Paired box 3 (PAX3), a transcription factor important in embryonic development, was found to mediate the formation of pericentromeric heterochromatin. However, how PAX3 recognizes the heterochromatic environment and re-establishes it after DNA replication remains unclear. MATERIALS AND METHODS: Cell-cycle synchronization, fluorescence microscopic analyses, and co-immunoprecipitation were used to analyze the heterochromatic localization of PAX3 in HEK 293 cells and NIH 3T3 cells. RESULTS: We found that PAX3 binds pericentromeric heterochromatin during middle-to-late S phase. Loading of PAX3 onto pericentromeric heterochromatin requires poly(ADP-ribose) polymerase 1 (PARP1). Furthermore, loss of PAX3 or PARP1 delays cell-cycle progression through the S phase. CONCLUSION: Our results reveal how PAX3 recognizes and maintains pericentromeric heterochromatin at the S phase of the cell cycle. Copyright© 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.
Relation:	ANTICANCER RESEARCH;34(9):4717-22.
Appears in Collections:	[生物科技學系] 期刊論文

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