Differential cytotoxic effects of gold nanoparticles in different mammalian cell lines

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題名:	Differential cytotoxic effects of gold nanoparticles in different mammalian cell lines
作者:	闕斌如(Chueh, Pin Ju)、Ruei-Yue Lia(Ruei-Yue Liang)、Yi-Hui Lee(Yi-Hui Lee)、Zih-Ming Zen(Zih-Ming Zeng)、Show-Mei Chu(Show-Mei Chuang)*
貢獻者:	生物科技學系
關鍵詞:	AuNPs;Cell-impedance measurement;Cytotoxicity;Gold nanoparticles;In vitro assays
日期:	201401
上傳時間:	2014-10-30 07:40:35 (UTC+0)
摘要:	Gold nanoparticles (AuNPs) possess unique properties that have been exploited in several medical applications. However, a more comprehensive understanding of the environmental safety of AuNPs is imperative for use of these nanomaterials. Here, we describe the impacts of AuNPs in various mammalian cell models using an automatic and dye-free method for continuous monitoring of cell growth based on the measurement of cell impedance. Several well-established cytotoxicity assays were also used for comparison. AuNPs induced a concentration-dependent decrease in cell growth. This inhibitory effect was associated with apoptosis induction in Vero cells but not in MRC-5 or NIH3T3 cells. Interestingly, cDNA microarray analyses in MRC-5 cells supported the involvement of DNA damage and repair responses, cell-cycle regulation, and oxidative stress in AuNP-induced cytotoxicity and genotoxicity. Moreover, autophagy appeared to play a role in AuNPs-induced attenuation of cell growth in NIH3T3 cells. In this study, we present a comprehensive overview of AuNP-induced cytotoxicity in a variety of mammalian cell lines, comparing several cytotoxicity assays. Collectively, these assays offer convincing evidence of the cytotoxicity of AuNPs and support the value of a systematic approach for analyzing the toxicology of nanoparticles. Copyright © 2013 Elsevier B.V. All rights reserved.
關聯:	JOURNAL OF HAZARDOUS MATERIALS;264:303-12.
顯示於類別:	[生物科技學系] 期刊論文

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