Identification of an Autoantibody against the Proliferating Cell Nuclear Antigen from a Patient with Systemic Lupus Erythematosus

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題名:	Identification of an Autoantibody against the Proliferating Cell Nuclear Antigen from a Patient with Systemic Lupus Erythematosus
作者:	Tsai-Ching Hsu;Joung-Liang Lan;Shao-Hsuan Kao;Sin-Lun Li;Yin-Chang Liu;Chih-Yang Huang;Bor-Show Tzang
貢獻者:	保健系
關鍵詞:	autoantibodies;systemic lupus erythematosus (SLE);proliferating cell nuclear antigen (PCNA);toroidal structure
日期:	2010-02
上傳時間:	2010-03-26 02:46:11 (UTC+0)
出版者:	Asia University
摘要:	Antibodies against the proliferating cell nuclear antigen (PCNA) was first discovered in the sera of systemic lupus erythematosus (SLE) patients. However, the reactivity and specificity of anti-PCNA autoantibodies are still unclear. To investigate the property of anti-PCNA autoantibodies, we conducted an ELISA screening of the anti-PCNA autoantibodies in sera of SLE patients. Eighteen out of 191 SLE sera were found to be positive for anti-PCNA antibodies giving a frequency of nearly 10%. Among the positive sera, a sample with the highest titer of anti-PCNA autoantibody preferentially recognizes the wild-type PCNA as compared to the Y114A mutation which contains a single amino acid substitution at 114 and fails to form the toroidal structure. Moreover, the autoantibody purified from this serum identifies only the free PCNA in crude mammalian cell extracts but not other associated cellular components. This finding raises a possibility that immunostaining with the human anti-PCNA autoantibodies in previous studies might have only partially PCNAs in tissues.
關聯:	CHINESE JOURNAL OF PHYSIOLOGY,53(1):11-18.
顯示於類別:	[食品營養與保健生技學系] 期刊論文

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