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| Title: | Berberine induced down-regulation of matrix metalloproteinase-1, -2 and -9 in human gastric cancer cells (SNU-5) in vitro |
| Authors: | Lin, JP (Lin, Jing-Pin);Yang, JS (Yang, Jai-Sing);Wu, CC (Wu, Chih-Chung);Lin, SS (Lin, Song-Shei);Hsieh, WT (Hsieh, Wen-Tsong);Lin, ML (Lin, Meng-Liang);Yu, FS (Yu, Fu-Shun);Yu, CS (Yu, Chun-Shu);Chen, GW (Chen, Guang-Wei);Chang, YH (Chang, Yung-Hsien);Chung, JG (Chung, Jing-Gung) |
| Contributors: | Department of Biotechnology |
| Keywords: | berberine;cell migration;NF-kappa B/p65;MMP-2;MMP-9;matrix metalloproteinase;MATRIX METALLOPROTEINASES;COLORECTAL TUMORIGENESIS;CRUDE EXTRACTS;HL-60 CELLS;CYCLIN B1;CARCINOMA;APOPTOSIS;INHIBITION;EXPRESSION;BERBERRUBINE |
| Date: | 2008-03 |
| Issue Date: | 2010-03-26 02:29:59 (UTC+0) |
| Publisher: | Asia University |
| Abstract: | Berberine, a yellow benzylisoquinoline alkaloid, is a constituent of Coptis chines and is commonly used in Chinese herbal medicine for patients with gastrointestinal disorders. The pharmacological effects of berberine include anti-inflammation, antidiarrhetic, antimalarial, and even antimicrobial activities. However, its mechanism of action on the cell migration of human gastric cancer SNU-5 cells is not fully understood. The effects of berberine on the percentage of viable cells were examined first and it was found that berberine induced dose-dependent inhibition in human gastric cancer SNU-5 cells. The effect of berberine on the levels of reactive oxygen species (ROS) and matrix metalloproteinase-1, -2, -7 and -9 was then examined using Western blotting and the results showed that berberine induced ROS production for up to 6 hours of incubation. It was also found that berberine induced down-regulation of MMP-1-2, and -9 but did not affect the level of MMP-7. The mRNA levels of MMPs in SNU-5 cells after treatment with berberine for 24 hours were investigated using a polymerase chain reaction and the results showed that berberine inhibited the gene expression of MMP-1, -2 and -9 in human SNU-5 cells but it did not affect MMP-7. In conclusion, berberine appears to exert its anticancer properties by inducing ROS production and prevention of cell migration via inhibition of the gene expression of MMP-1, -2 and -9 in human gastric cancer SNU-5 cancer cells. |
| Relation: | IN VIVO, 22 (2): 223-230 |
| Appears in Collections: | [Department of Biotechnology] Journal Article
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