Diallyl disulfide induces apoptosis in human colon cancer cell line (COLO 205) through the induction of reactive oxygen species, endoplasmic reticulum stress, caspases casade and mitochondrial-dependent pathways

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题名:	Diallyl disulfide induces apoptosis in human colon cancer cell line (COLO 205) through the induction of reactive oxygen species, endoplasmic reticulum stress, caspases casade and mitochondrial-dependent pathways
作者:	Yang, JS (Yang, Jai-Sing);Chen, GW (Chen, Guang-Wei);Hsia, TC (Hsia, Te-Chun);Ho, HC (Ho, Heng-Chien);Ho, CC (Ho, Chin-Chin);Lin, MW (Lin, Meng-Wei);Lin, SS (Lin, Song-Shei);Yeh, RD (Yeh, Ru-Duan);Ip, SW (Ip, Siu-Wan);Lu, HF (Lu, Hsu-Fung);Chung, JG (Chung, Jing-Gung)
贡献者:	Department of Biotechnology
关键词:	Diallyl disulfide;Caspase-3;Apoptosis;Mitochondria;Reactive oxygen species;cDNA microarray
日期:	2009-01
上传时间:	2010-03-26 02:29:37 (UTC+0)
出版者:	Asia University
摘要:	In this study, we investigated the effects of DADS on human colon cancer cell line COLO 205 on cell cycle arrest and apoptosis in vitro. After 24 h treatment of COLO 205 cells with DADS, the dose- and time-dependent decreases of viable cells were observed and the IC50 was 22.47 mu M. The decreased percentages of viable cells are associated with the production of ROS. Treatment of COLO 205 cells with DADS resulted in G2/M phase arrest and apoptosis occurrence through the mitochondrial-pathway (Bcl-2, Bcl-xL down-regulation and Bak, Bax up-regulation). DADS increased cyclin B, cdc25c-ser-216-9 and Weel but did not affect CDK1 protein and gene expression within 24 h of treatment. DADS-induced apoptosis was examined and confirmed by DAPI staining and DNA fragmentation assay. DADS promoted caspase-3, -8 and -9 activity and induced apoptosis were accompanied by increasing the levels of Fas, phospho-Ask1 and JNK, p53 and decreasing the mitochondrial membrane potential which then led to release the cytochrome c, cleavage of pro-caspase-9 and -3. The COLO 205 cells were pre-treated with JNK inhibitor before leading to decrease the percentage of apoptosis which was induced by DADS. Inhibition of caspase-3 activation blocked DADS-induced apoptosis on COLO 205 cells.
關聯:	FOOD AND CHEMICAL TOXICOLOGY 47 (1): 171-179
显示于类别:	[生物科技學系] 期刊論文

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