Baicalein-Induced Apoptosis via Endoplasmic Reticulum Stress Through Elevations of Reactive Oxygen Species and Mitochondria Dependent Pathway in Mouse-Rat Hybrid Retina Ganglion Cells (N18)

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/8288

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://asiair.asia.edu.tw/ir/handle/310904400/8288

题名:	Baicalein-Induced Apoptosis via Endoplasmic Reticulum Stress Through Elevations of Reactive Oxygen Species and Mitochondria Dependent Pathway in Mouse-Rat Hybrid Retina Ganglion Cells (N18)
作者:	Li, YC (Li, Yu-Ching);Lin, HJ (Lin, Hui-Ju);Yang, JH (Yang, Jen-Hung);Yang, JS (Yang, Jai-Sing);Ho, HC (Ho, Heng-Chien);Chang, SJ (Chang, Shu-Jen);Hsai, TC (Hsai, Te-Chun);Lu, HF (Lu, Hsu-Feng);Huang, AC (Huang, An-Cheng);Chung, JG (Chung, Jing-Gung)
贡献者:	Department of Biotechnology
关键词:	Baicalein;Reactive oxygen species (ROS);Cytochrome c;Cytoplasmic Ca2+;Caspase-3;Mitochondrial death pathway;1,2-Bis(2-aminophenoxy) ethane-N,N,N ',N '-tetraacetic acid (BAPTA);Apoptosis
日期:	2009-03
上传时间:	2010-03-26 02:29:35 (UTC+0)
出版者:	Asia University
摘要:	Studies were designed to investigate the effects of baicalein on mouse-rat hybrid retina ganglion cells (N18) to better understand its effect on apoptosis and apoptosis-related genes in vitro. Cell viability, reactive oxygen species (ROS), cytoplasmic Ca2+, mitochondrial membrane potential (MMP), apoptosis induction, and caspases-3 activity were examined by flow cytometric assay. Apoptosis-associated proteins such as p53, Bax, Bcl-2, cytochrome c, and caspase-3 were examined by Western blot. We demonstrated the increase in the levels of p53, Bax, and cytochrome c and decrease in the level of Bcl-2, which are associated with the induction of apoptotic cell death after 24 h treatment with baicalein in N18 cells. Baicalein induced an increase in the cytoplasmic levels of ROS and Ca2+ in 1 h and reached their peak at 3 h, and thereafter a loss of MMP by flow cytometry. We also demonstrated a release of the cytochrome c from mitochondria into cytosol and an activation of caspase-3, which led to the occurrence of apoptosis in N18 cells treated with baicalein by Western blot. Pretreatment was conducted with BAPTA (intracellular calcium chelator) in baicalein-treated cells, the decline of MMP was recovered, and the increase in the level of cytoplasmic Ca2+ was suppressed, and the proportion of apoptosis was also markedly diminished. In conclusion, our data suggests that oxidative stress and cellular Ca2+ modulates the baicalein-induced cell death via a Ca2+-dependent mitochondrial death pathway in N18 cells.
關聯:	NEUROCHEMICAL RESEARCH 34 (3): 418-429
显示于类别:	[生物科技學系] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
		0Kb	Unknown	473	检视/开启
183.doc		37Kb	Microsoft Word	229	检视/开启

在ASIAIR中所有的数据项都受到原著作权保护.

数据加载中.....