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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/8281


    Title: Detection of Conjugated Soy Metabolites in Urinary and Tissue Samples after Methanol Extraction
    Authors: Chang, HC (Chang, Hebron C.);Han, J (Han, Jiayuan);Prior, RL (Prior, Ronald L.)
    Contributors: Department of Biotechnology
    Keywords: methanol pretreatment;urine;tissue;glucuronide conjugate
    Date: 2009-02
    Issue Date: 2010-03-26 02:29:32 (UTC+0)
    Publisher: Asia University
    Abstract: Glucuronide conjugates of soy isoflavone metabolites genistein, daidzein, glycitein and equol in urinary samples from women, female pigs and female rats were pretreated with methanol instead of solid phase extraction or liquid-liquid extraction after enzymatic digestion. The samples were analyzed using an LC-ESI-MS/MS ion trap system. Analysis of rat liver sample using this procedure was also attempted. By comparison the elution sequences with results from previous studies, glucuronide conjugates including 7-gluc-Dai, 7-gluc-Gly, 4'-gluc-Dai, 7-gluc-Gen, then 4'-gluc-Gen were identified sequentially in women urine. However, 5-glue-Gen was also observed in female porcine urine. In rat urine, sul-Dai was observed after 7-gluc-Dai, while 7-gluc-Equ and 4'-gluc-Equ were observed after 7-gluc-Gen. The metabolite profiles in rat liver tissue samples were found with 7-gluc-Dai and 7-gluc-Gen. However, glucuronide conjugates of equol and sulfate conjugate of daidzein were only found in rat urine. Interestingly, quantification studies performed by LC-APCI/MS and LC-ECD showed that the aglycone and conjugated glycitein were both found in porcine and rat urine, but only conjugated glycitein was found in woman urine.
    Relation: JOURNAL OF FOOD AND DRUG ANALYSIS 17 (1): 43-51
    Appears in Collections:[生物科技學系] 期刊論文

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