AmpD(I) Is Involved in Expression of the Chromosomal L1 and L2 beta-Lactamases of Stenotrophomonas maltophilia

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/8268

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Title:	AmpD(I) Is Involved in Expression of the Chromosomal L1 and L2 beta-Lactamases of Stenotrophomonas maltophilia
Authors:	Yang, TC (Yang, Tsuey-Ching);Huang, YW (Huang, Yi-Wei);Hu, RM (Hu, Rouh-Mei);Huang, SC (Huang, Shao-Cheng);Lin, YT (Lin, Yu-Tzu)
Contributors:	Department of Biotechnology
Keywords:	L-ALANINE AMIDASE;PSEUDOMONAS-AERUGINOSA;CITROBACTER-FREUNDII;ESCHERICHIA-COLI;ENTEROBACTER-CLOACAE;AMPC;GENE;INDUCTION;CEPHALOSPORINASE;OVERPRODUCTION
Date:	2009-07
Issue Date:	2010-03-26 02:29:27 (UTC+0)
Publisher:	Asia University
Abstract:	Two ampD homologues, ampD(I) and ampD(II), of Stenotrophomonas maltophilia have been cloned and analyzed. Comparative genomic analysis revealed that the genomic context of the ampD(II) genes is quite different, whereas that of the ampD(I) genes is more conserved in S. maltophilia strains. The ampD system of S. maltophilia is distinct from that of the Enterobacteriaceae and Pseudomonas aeruginosa in three respects. (i) AmpD(I) of S. maltophilia is not encoded in an ampDE operon, in contrast to what happens in the Enterobacteriaceae and P. aeruginosa. (ii) The AmpD systems of the Enterobacteriaceae and P. aeruginosa are generally involved in the regulation of ampR-linked ampC gene expression, while AmpD(I) of S. maltophilia is responsible for the regulation of two intrinsic beta-lactamase genes, of which the L2 gene, but not the L1 gene, is linked to ampR. (iii) S. maltophilia exhibits a one-step L1 and L2 gene derepression model involving ampD(I), distinct from the two-or three-step derepression of the Enterobacteriaceae and P. aeruginosa. Moreover, the ampD(I) and ampD(II) genes are constitutively expressed and not regulated by the inducer and AmpR protein, and the expression of ampD(II) is weaker than that of ampD(I). Finally, AmpD(II) is not associated with the derepression of beta-lactamases, and its role in S. maltophilia remains unclear.
Relation:	ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 53 (7): 2902-2907
Appears in Collections:	[生物科技學系] 期刊論文

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