The effect of temperature on the equilibrium adsorption of Methylene Blue dye from aqueous solution using bentonite was investigated. The equilibrium adsorption data were analyzed using three widely applied isotherms: Langmuir, Freundlich, and Redlich-Peterson. A non-linear method was used for comparing the best fit of the isotherms. Best fit was found to be Redlich-Peterson isotherm. Thermodynamic parameters, such as Delta G degrees, Delta H degrees, and Delta S degrees, were calculated using adsorption equilibrium constant obtained from the Langmuir isotherm. Results suggested that the Methylene Blue adsorption on bentonite was a spontaneous and endothermic process.