Involvement of Matrix Metalloproteinases in the Inhibition of Cell Invasion and Migration Through the Inhibition of NF-kappa B by the New Synthesized Ethyl 2-[N-p-chlorobenzyl-(2'-methyl)]anilino-4-oxo-4,5-dihydrofuran-3-carboxylate (JOTO1007) in Human Cervical Cancer Ca Ski Cells

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/8261

請使用永久網址來引用或連結此文件: http://asiair.asia.edu.tw/ir/handle/310904400/8261

題名:	Involvement of Matrix Metalloproteinases in the Inhibition of Cell Invasion and Migration Through the Inhibition of NF-kappa B by the New Synthesized Ethyl 2-[N-p-chlorobenzyl-(2'-methyl)]anilino-4-oxo-4,5-dihydrofuran-3-carboxylate (JOTO1007) in Human Cervical Cancer Ca Ski Cells
作者:	Huang, AC (Huang, An-Cheng);Hsu, SC (Hsu, Shu-Chun);Kuo, CL (Kuo, Chao-Lin);Liao, CL (Liao, Ching-Lung);Lai, KC (Lai, Kuang-Chi);Lin, TP (Lin, Tsung-Ping);Wu, SH (Wu, Shin-Hwar);Lu, HF (Lu, Hsu-Feng);Tang, NY (Tang, Nou-Ying);Yang, JS (Yang, Jai-Sing);Chung, JG (Chung, Jing-Gung)
貢獻者:	Department of Biotechnology
關鍵詞:	JOTO1007;invasion;migration;MMPs;Ca Ski cells;CARCINOMA CELLS;GROWTH;APOPTOSIS;DYNAMICS;MOTILITY;PATHWAY
日期:	2009-07
上傳時間:	2010-03-26 02:29:25 (UTC+0)
出版者:	Asia University
摘要:	JOTO1007 (ethyl 2-[N-p-chlorobenzyl-(2'-methyl)] anilino-4-oxo-4,5-dihydrofuran-3-carboxylate) has anticancer effects in human cervical cancer Ca Ski cells. However, its mechanism of action on the cell migration and invasion of human cervical cancer Ca Ski cells is not fully understood. In this study, firstly, the effects of JOTO1007 on the migration and invasion of Ca Ski cells were examined by using matrigel counting. The results showed that JOT01007 suppressed the migration and invasion of the Ca Ski cells. Secondly, the effect of JOTO1007 on the levels of proteins associated with cell metastasis was examined using Western blotting. The results indicated that JOTO1007 inhibited the levels of son of sevenless homolog 1 (SOS-1), growth factor receptor-bound protein 2 (GRB2), Ras homolog gene family, member A (RhoA), Rho-associated, coiled-coil containing protein kinase 1 (ROCK-1), focal adhesion kinase (FAK), phosphorylated-c-jun (p-c-jun), nuclear factor kappa B (NF-kappa B) p65, cyclooxygenase-2 (COX-2), extracellular signal-regulated kinases 1/2 (ERK1/2), matrix metalloproteinase-2 (MMP-2), MMP-7 and MMP-9, but promoted the levels of protein kinase C (PKC), phosphoinositide 3-kinases (PI3K), MAP kinase kinase kinase 3 (MEKK-3), mitogen-activated protein kinase kinase 7 (MKK7), c-jun and inducible nitric oxide synthases (iNOS), while not affecting Ras, phosphorylated-ERK (p-ERK), p38 and c-jun N-terminal kinase 1/2 (JNK1/2), which finally led to the inhibition of migration and invasion of the Ca Ski cells in vitro. Overall, JOTO1007 inhibited NF-kappa B which then led to the inhibition of the MMP-2, -7 and -9 expression followed by the inhibition of migration and invasion in the Ca Ski cells.
關聯:	IN VIVO 23 (4): 613-619
顯示於類別:	[生物科技學系] 期刊論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
156.doc		38Kb	Microsoft Word	234	檢視/開啟
index.html		0Kb	HTML	370	檢視/開啟

在ASIAIR中所有的資料項目都受到原著作權保護.

資料載入中.....