Polylactic acid (PLA) is a synthetic polymer that has biocompatibility and biodegradation. This study aims to examine the influence of manufacturing parameters on the physical properties of tubular PLA/Spandex/Gelatin composite braids. PLA fibers and spandex are combined, braided and then immersed in gelatin solution to form tubular PLA/Spandex/Gelatin composite braids. The tensile strength, bursting strength, and porosity of the resulting braids are tested, and the experiment results show that a greater twist per inch (T.P.I.) causes a higher tensile strength and bursting strength, but does not change the porosity distinctively.