Determination of lupeol in common medicinal plants for clearing heat and detoxication in Taiwan by high performance liquid chromatography

ASIA unversity > 醫學暨健康學院 > 食品營養與保健生技學系 > 博碩士論文 > Item 310904400/80717

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題名:	Determination of lupeol in common medicinal plants for clearing heat and detoxication in Taiwan by high performance liquid chromatography
作者:	Yen, Yu-Hsin
貢獻者:	保健營養生技學系
關鍵詞:	lupeol HPLC learing heat and detoxication
日期:	2014-07-30
上傳時間:	2014-09-03 07:07:59 (UTC+0)
出版者:	Asia University
摘要:	A method first developed to determinated of lupeol (a novel anti-inflammatory and anti-cancer dietary triterpene) contained in 46 kinds of common medicinal plants in Taiwan was established. The samples were analyzed by HPLC on a Thermo ODS-C18 Column (250 × 4.6 mm，5 μm) and detected at 210 nm with acetonitrile water (99:1, v/v) as the mobile phase at a flow rate of 1.0 mL/min. The regression equations of diosgenin was Y = 4.612 X - 9.118 (R = 0.999). The intraday and interday relative standard deviations of lupeol were at the levels of 1.34-3.87% and 0.47-2.38%, respectively. percent recovery values of lupeol were at the levels of 89.00-103.42%. Detection limit was 50 μg/mL based on a signal-to-noise ratio of 3:1. The method was applied to 46 samples and lupeol was detected in 5 samples, measurable at 192.4 mg/g contained in Acanthopanax trifoliatus (sequential extracts of n-hexane), 29.3 mg/g contained in Elephantopus mollis (sequential extracts of n-hexane), 1.4 mg/g contained in Elephantopus mollis (methanol extracts), 10.1 mg/g contained in Pellionia radicans (methanol extracts), 609.0 μg/g (Dry weight) contained in Taraxacum officinale root。In conclusion, this study had shown that the HPLC method could be applied successfully to analyze lupeol contained in common medicinal plants. Therefore, an easy and suitable method for the detection and assay of lupeol was established.
顯示於類別:	[食品營養與保健生技學系] 博碩士論文

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