Alteration of FSH-stimulated progesterone production and calcium homeostasis in primarily cultured human luteinizing-granulosa cells induced by Fenvalerate.

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Title:	Alteration of FSH-stimulated progesterone production and calcium homeostasis in primarily cultured human luteinizing-granulosa cells induced by Fenvalerate.
Authors:	J. He;J. F. Chen;R. Liu;S. L. Wang;L. Song;H. C. Chang;X. R. Wang
Contributors:	Department of Biotechnology
Keywords:	Endocrine disruptor;Pyrethroids;Granulosa cell;Progesterone;Calcium homeostasis
Date:	2004
Issue Date:	2010-03-15 08:11:59 (UTC+0)
Publisher:	Asia University
Abstract:	Fenvalerate, a synthetic pyrethroid, is widely used in agriculture and other domestic applications in China. Recently, Fenvalerate has been suspected to be one of the endocrine-disrupting chemicals (EDC). In this study, we investigated the effects of fenvalerate on follicle-stimulating hormone (FSH)-stimulated progesterone (P4) production by human ovarian luteinizing-granulosa cells (hGLCs). After 24 h incubation, fenvalerate inhibited FSH-stimulated P4 production. At the same time, FSH-stimulated cAMP also decreased. Due to calcium and Ca2+–calmodulin (CaM) system involving gonadotropin-stimulated steroidogenesis by granulosa cells, we then evaluated the effects of fenvalerate on trifluoperazine (TFP)- and verapamil-driven FSH-stimulated P4 production. The results showed that calcium or calmodulin might play a role in fenvalerate-induced alterations in FSH-stimulated P4 biosysthesis. Then, the effects of fenvalerate on calcium homeostasis in hGLCs were studied. The result showed that 5 μM fenvalerate induced a slow increase in [Ca2+]i in hGLCs by using a fluorescent Ca2+ indicator fluo-3/AM. The changes in total concentration of CaM in hGLCs induced by fenvalerate were evaluated by a method of immunofluorescence. There is a significant increase in all treated groups. In summary, fenvalerate could inhibit FSH-stimulated P4 production. Also, fenvalerate interferes with calcium homeostasis in hGLCs. The effects of fenvalerate on FSH-stimulated ovarian steroidogenesis may be mediated partly through calcium signal.
Relation:	Toxicology 203: 61-68
Appears in Collections:	[Department of Biotechnology] Journal Article

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