The Role of AmpR in the Regulation of L1 and L2 b-lactamases in Stenotrophomonas maltophilia

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Title:	The Role of AmpR in the Regulation of L1 and L2 b-lactamases in Stenotrophomonas maltophilia
Authors:	Cheng-Wen Lin;Yi-Wei Huang;Rouh-Mei Hu;Kai-Hung Chiang;Tsuey-Ching Yang
Contributors:	Department of Biotechnology
Keywords:	Stenotrophomonas maltophilia;β-lactamase;AmpR
Date:	2008
Issue Date:	2010-03-15 08:11:36 (UTC+0)
Publisher:	Asia University
Abstract:	Stenotrophomonas maltophilia is known to produce at least two chromosomal-mediated inducible β-lactamases, L1 and L2. Gene L2, which encodes a class A β-lactamase, and the adjacent ampR gene form an ampR-class A β-lactamase module. L1 belongs to the class B β-lactamase and has no neighbor ampR-like regulatory gene. In this study, the ampR-L2 module from S. maltophilia KH was compared with ampR-β-lactamase modules from several microorganisms with respect to the AmpR and β-lactamase proteins and the intergenic (IG) region. S. maltophilia and Xanthomonas campestris showed the most closely phylogenetic relationship among the microorganisms considered. The regulatory role of AmpR towards L1 and L2 was further analyzed. In the absence of an inducer, AmpR acted as an activator for L1 expression and as a repressor for L2 expression, whereas AmpR was an activator for both genes in an induced state. In addition, inducibility of L1 and L2 genes depended on the presence of AmpR. The ampR transcript was weakly and constitutively expressed, but was not autoregulated.
Relation:	Research in Microbiology,160(2),152–158.
Appears in Collections:	[Department of Biotechnology] Journal Article

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