Regulation of the pehA Gene Encoding the Major Polygalacturonase of Xanthomonas campestris by Clp and RpfF

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Title:	Regulation of the pehA Gene Encoding the Major Polygalacturonase of Xanthomonas campestris by Clp and RpfF
Authors:	Yi-Min Hsiao;Mei-Huei Zheng;Rouh-Mei Hu;Tsuey-Ching Yang;Yi-Hsiung Tseng
Contributors:	Department of Biotechnology
Date:	2008-03
Issue Date:	2010-03-15 08:11:33 (UTC+0)
Publisher:	Asia University
Abstract:	Exopolysaccharide and several extracellular enzymes of Xanthomonas campestris pv. campestris (Xcc), the causative agent of black rot in crucifers, are virulence factors. In this study, sequence and mutational analysis has demonstrated that Xcc pehA encodes the major polygalacturonase, a member of family 28 of the glycosyl hydrolases. Using the 5' RACE (rapid amplification of cDNA ends) method, the pehA transcription initiation site was mapped at 102 nt downstream of a Clp (cAMP receptor protein-like protein)-binding site. Transcriptional fusion assays showed that pehA transcription is greatly induced by polygalacturonic acid, positively regulated by Clp and RpfF (an enoyl-CoA hydratase homologue which is required for the synthesis of cis-11-methyl-2-dodecenoic acid, a low-molecular-mass diffusible signal factor), subjected to catabolite repression, which is independent of Clp or RpfF, and repressed under conditions of oxygen limitation or nitrogen starvation. Our findings extend previous work on Clp and RpfF regulation to show that they both influence the expression of pehA in Xcc.
Relation:	Microbiology 154(Pt 3):705-13
Appears in Collections:	[Department of Biotechnology] Journal Article

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