Rat skeletal muscle selenoprotein W: cDNA clone and mRNA modulation by dietary selenium

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Title:	Rat skeletal muscle selenoprotein W: cDNA clone and mRNA modulation by dietary selenium
Authors:	Vendel, S. C.;Beilstein, M. A.;Yeh, J. Y.;Ream, L. W.;Whanger, P. D
Contributors:	Department of Biotechnology
Date:	1995
Issue Date:	2010-03-15 08:10:57 (UTC+0)
Publisher:	Asia University
Abstract:	Rat skeletal muscle selenoprotein W cDNA was isolated and sequenced. The isolation strategy involved design of degenerate PCR primers from reverse translation of a partial peptide sequence. A reverse transcription-coupled PCR product from rat muscle mRNA was used to screen a muscle cDNA library prepared from selenium-supplemented rats. The cDNA sequence confirmed the known protein primary sequence, including a selenocysteine residue encoded by TGA, and identified residues needed to complete the protein sequence. RNA folding algorithms predict a stem-loop structure in the 3' untranslated region of the selenoprotein W mRNA that resembles selenocysteine insertion sequence (SE-CIS) elements identified in other selenocysteine coding cDNAs. Dietary regulation of selenoprotein W mRNA was examined in rat muscle. Dietary selenium at 0.1 ppm as selenite increased muscle mRNA 4-fold relative to a selenium-deficient diet. Higher dietary selenium produced no further increase in mRNA levels.
Relation:	Proceedings of the National Academy of Sciences of the United States of America 92:8749-8753
Appears in Collections:	[Department of Biotechnology] Journal Article

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