Cloning and Characterization of a Plant Defensin VaD1 from Azuki Bean.

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/7963

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://asiair.asia.edu.tw/ir/handle/310904400/7963

题名:	Cloning and Characterization of a Plant Defensin VaD1 from Azuki Bean.
作者:	Gan-Hong Chen;Ming-Pin Hsu;Chi-Hsing Tan;Hsien-Yi Sung;Ming-Jen Fan;Huei-Mei Chen;Ching-San Chen
贡献者:	Department of Biotechnology
日期:	2005
上传时间:	2010-03-15 08:10:48 (UTC+0)
出版者:	Asia University
摘要:	A recombinant mungbean defensin VrD1 was previously shown to exhibit antifungal and bruchid-resistant activity. To study the function and regulation of VrD1, genomic DNAs of plant defensins were isolated from Vigna radiata VC6089A and azuki bean Vigna angularis Kao Hsiung No. 6. The azuki bean defensin genomic DNA VaD1 was sequenced and converted to VaD1 cDNA. VaD1 defensin was purified from Vigna angularis Kao Hsiung No. 6 to apparent homogeneity. The complete amino acid sequence of the purified VaD1 was determined and was found to be exactly the same as the sequence deduced from VaD1 cDNA. VaD1 is a basic protein containing 46 amino acids with four conserved disulfide bonds and shares high sequence homology (78.3%) with VrD1. VaD1 inhibited the growth of Fusarium oxysporum, Fusarium oxysporum f. sp. pisi, Staphylococcus epidermidis, and Salmonella typhimurium. VaD1 also inhibited in vitro protein synthesis and bruchid larval development, but was less active than the recombinant VrD1.
關聯:	Journal of Agricultural and Food Chemistry 53:982-988
显示于类别:	[生物科技學系] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
		0Kb	Unknown	383	检视/开启
310904400-7963.doc		30Kb	Microsoft Word	178	检视/开启

在ASIAIR中所有的数据项都受到原著作权保护.