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    Please use this identifier to cite or link to this item: http://asiair.asia.edu.tw/ir/handle/310904400/79604


    Title: Affinity adsorption of lysozyme with Reactive Red 120 modified magnetic chitosan microspheres
    Authors: Zhonghong Li;Miao Cao;Wengang Zhang;Lizhi Liu;Jianlong Wang;Wupeng Ge;Yahong Yuan;Tianli Yue;Ronghua Li;William W. Yu
    Contributors: 生物科技學系
    Keywords: Adsorption;Egg white;Lysozyme;Magnetic microspheres;Reactive Red 120
    Date: 2014-05
    Issue Date: 2014-06-04 02:24:28 (UTC+0)
    Abstract: Reactive Red 120 was utilized as an affinity dye-ligand to modify the surface of magnetic chitosan microspheres to improve the adsorption capacity for lysozyme. The impact of pH, ionic strength, adsorption time and initial lysozyme concentration on the adsorption of lysozyme from aqueous solutions was investigated. An increased adsorption amount of 116.9 mg/g lysozyme on Reactive Red 120 modified microspheres was achieved in comparison to 24.6 mg/g on unmodified magnetic chitosan microspheres. The adsorption behaviour of lysozyme by the Reactive Red 120 modified magnetic chitosan microspheres fitted the pseudo second-order kinetic and the Langmuir thermodynamic model. The desorption ratio was 92.6% using 1M NaCl phosphate buffer (pH 6.0) as the desorption agent. The adsorption capacity of the Reactive Red 120 modified magnetic chitosan microspheres did not decrease significantly after four adsorption-desorption cycles (P>0.05). The as-prepared Reactive Red 120 modified magnetic chitosan microspheres were then used for the extraction of lysozyme from egg white solutions. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) results showed that the purity of the obtained lysozyme was ~80.7% and that the recovery yield was ~89.1%.
    Copyright © 2013 Elsevier Ltd. All rights reserved.
    Relation: FOOD CHEMISTRY,145:749-755
    Appears in Collections:[生物科技學系] 期刊論文

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