Broad-spectrum transgenic resistance against distinct tospovirus species at the genus level

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Title:	Broad-spectrum transgenic resistance against distinct tospovirus species at the genus level
Authors:	彭瑞菊;Peng, J.-C.;陳宗祺;Chen, Tsung-Chi;Raja, J.A.;Raja, J.A.J.;楊景富;Yang, C.-F.;簡婉竹;Chien, W.-C.;林貞璇;Lin, C.-H.;劉芳麟;Liu, F.-L.;吳惠雯;Wu, H.-W.;葉錫東;Yeh, S.-D.
Contributors:	生物科技學系
Date:	2014-03
Issue Date:	2014-06-04 02:20:01 (UTC+0)
Abstract:	Thrips-borne tospoviruses cause severe damage to crops worldwide. In this investigation, tobacco lines transgenic for individual WLm constructs containing the conserved motifs of the L RNA-encoded RNA-dependent RNA polymerase (L) gene of Watermelon silver mottle virus (WSMoV) were generated by Agrobacterium-mediated transformation. The WLm constructs included: (i) translatable WLm in a sense orientation; (ii) untranslatable WLmt with two stop codons; (iii) untranslatable WLmts with stop codons and a frame-shift; (iv) untranslatable antisense WLmA; and (v) WLmhp with an untranslatable inverted repeat of WLm containing the tospoviral S RNA 3'-terminal consensus sequence (5'-ATTGCTCT-3') and an NcoI site as a linker to generate a double-stranded hairpin transcript. A total of 46.7-70.0% transgenic tobacco lines derived from individual constructs showed resistance to the homologous WSMoV; 35.7-100% plants of these different WSMoV-resistant lines exhibited broad-spectrum resistance against four other serologically unrelated tospoviruses Tomato spotted wilt virus, Groundnut yellow spot virus, Impatiens necrotic spot virus and Groundnut chlorotic fan-spot virus. The selected transgenic tobacco lines also exhibited broad-spectrum resistance against five additional tospoviruses from WSMoV and Iris yellow spot virus clades, but not against RNA viruses from other genera. Northern analyses indicated that the broad-spectrum resistance is mediated by RNA silencing. To validate the L conserved region resistance in vegetable crops, the constructs were also used to generate transgenic tomato lines, which also showed effective resistance against WSMoV and other tospoviruses. Thus, our approach of using the conserved motifs of tospoviral L gene as a transgene generates broad-spectrum resistance against tospoviruses at the genus level.
Relation:	PLoS One
Appears in Collections:	[Department of Biotechnology] Journal Article

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