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| Title: | The anti-tumor activity of Antrodia salmonea in human promyelocytic leukemia;HL-60) cells is mediated via the induction of G1 cell-cycle arrest and apoptosis in vitro or in vivo |
| Authors: | 許游章;Hseu, You-Cheng;李傳珍;Lee, Chuan-Chen;Yung-Chang, C;Chen, Yung-Chang;K.J.Senthil;Kumar, K.J.Senthil;Ch, Chee-Shan;Chen, Chee-Shan;Huang, Yu-Chi;Huang, Yu-Chi;Hsu, Li-Sung;Hsu, Li-Sung;Huan, Hui-Chi;Huang, Hui-Chi;Ya, Hsin-Ling;*, Hsin-Ling Yang |
| Contributors: | 保健營養生技學系 |
| Keywords: | Antrodia salmonea;Human promyelocytic leukemia;G1 cell-cycle arrest;Apoptosis;Xenografted nude mice |
| Date: | 2014-04 |
| Issue Date: | 2014-06-03 11:22:57 (UTC+0) |
| Abstract: | Ethnopharmacological relevance
The medicinal mushroom Antrodia salmonea has been used as a traditional Chinese medicine and has demonstrated antioxidant and anti-inflammatory effects.
Materials and methods
In the present study, we examined the anti-tumor activity of the fermented culture broth of Antrodia salmonea (AS) in vitro and in vivo and revealed its underlying molecular mechanism of action.
Results
Treatment of human promyelocytic leukemia (HL-60) cells with AS (50–150 μg/mL) significantly reduced cell viability and caused G1 arrest via the inhibition of cell-cycle regulatory proteins, including cyclin D1, CDK4, cyclin E, cyclin A, and phosphorylated retinoblastoma protein (p-Rb). Furthermore, AS treatment induced apoptosis, which was associated with DNA fragmentation, followed by a sequence of events, including intracellular ROS generation; mitochondrial dysfunction; Fas ligand activation; cytochrome c release; caspase-3, -8, -9, and PARP activation; and Bcl-2/Bax dysregulation. The results of the in vitro study suggested that AS-induced apoptosis in HL-60 cells was mediated by both the mitochondrial and death receptor pathways. Furthermore, we found that AS treatment was effective in delaying tumor incidence in HL-60 xenografted nude mice and reducing tumor burden.
Conclusions
To the best of our knowledge, this is the first report confirming the anti-tumor activity of this potentially beneficial mushroom against human promyelocytic leukemia. |
| Relation: | JOURNAL OF ETHNOPHARMACOLOGY,153 (2014),499–510. |
| Appears in Collections: | [食品營養與保健生技學系] 期刊論文
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