Cytotoxicity of venom from crown-of-thorns starfish (Acanthaster planci) spine

ASIA unversity > 醫學暨健康學院 > 食品營養與保健生技學系 > 期刊論文 > Item 310904400/79193

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題名:	Cytotoxicity of venom from crown-of-thorns starfish (Acanthaster planci) spine
作者:	黃登福;Hwang, Deng-Fwu
貢獻者:	保健營養生技學系
日期:	2013-12
上傳時間:	2014-01-06 12:20:42 (UTC+0)
摘要:	The cytotoxic effects of the crown-ofthorns starfish Acanthaster planci spine venom (ASV) in five cell lines, including human neuroblastoma (SHSY5Y), human hepatocellular carcinoma (HepG2), human melanoma (A375.S2), human skin fibroblast (CCD-966SK) and mouse macrophage-like cell (RAW 264.7) were assayed. The results indicated that ASV showed cytotoxic effects depending on dose in these five cell lines. Specifically, ASV significantly inhibited the proliferation of human melanoma cell line A375.S2 at 10 μg/mL, indicating A. planci spine venom could be utilized as potential chemotherapeutic agent in the treatment of cancer. The cytotoxicity of ASV to human melanoma cell line A375.S2 was relatively well retained at temperature less than 40°C, and sharply lost at temperature more than 80°C. The cytotoxicity of ASV also sharply lost at extreme pH environments (pH 2 and 12). The cytotoxicity of ASV was attenuated when treated with Cu2+ and anti-oxidant N-acetylcysteine. After SDS-PAGE analysis, ASV showed the major protein components ranging from 10 kDa to 37 kDa.
關聯:	Molecular & Cellular Toxicology; 9(2):177-184
顯示於類別:	[食品營養與保健生技學系] 期刊論文

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