ASIA unversity:Item 310904400/7800
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    题名: Identification of species and measurement of tetrodotoxin in dried dressed fillets of the puffer fish, Lagocephalus lunaris
    作者: Chen, T. Y.;Y. W. Hsieh;Y. H. Tsai;C. Y. Shiau;D. F. Hwang
    日期: 2002
    上传时间: 2010-02-26
    出版者: Asia University
    摘要: The toxicity and species of dried dressed fish fillets were investigated. Three of seven dried dressed fish fillets were found to be toxic, with their levels of toxicity ranging from 9 to 18 mouse units per g. The toxin preparation was further purified and identified as tetrodotoxin and anhydrotetrodotoxin by high-performance liquid chromatography and gas chromatography-mass spectroscopy. The species of these fillets could not be distinguished from Lagocephalus lunaris by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis or restriction site and sequence analysis of a polymerase chain reaction amplicon of the cytochrome b gene. These fish fillets were identified as L. lunaris on the basis of their protein patterns and gene characteristics. Furthermore, the toxic samples contained low levels of tetrodotoxin and its derivative.
    關聯: Journal of Food Protection 65:1670-1673
    显示于类别:[食品營養與保健生技學系] 期刊論文

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