全球基因轉殖作物的栽培正蓬勃發展，支持與反對基因轉殖的論述彼此爭論不休，建立可辨識基因轉殖或非基因轉殖生物的檢測方法，以讓大眾能有知道權利及選擇性是非常需要的。本研究的主要研究目的，為建立能基因轉殖作物外來基因(foreign gene)的BLAST 資料庫，以提供快速的序列同源性比對，提供做為基因轉殖作物檢測系統所需的資訊。本研究以基因轉殖作物外來基因(foreign gene)為研究對象，利用AGBIOS 之GM Database 取得已通過評估可釋放於環境之GM 作物外來基因等資訊，且以BioPerl 設計操作指令群對NCBI Genbank 送出字串查詢核酸序列，以自動取得相關的核酸序列建立本機BLAST 資料庫，使用B2D Linux Server 平台及WWWBLAST 介面，提供國內研究人員快速且有效率的比對出所研究的序列是否為GMO 外來基因。本研究建已立特定基因之GMO BLAST 資料庫，並且可自行擴充資料庫，可於網際網路使用。The cultivation area in global scare of transgenic crop is increased rapidly. The open ion between support and opposition about the GMO is argued continuously. The mythos to recognize the GMO and NON-GMO are needed very necessary for consumer’s right.
The goal of this research is to constructed a GMO BLAST Database system which can provide rapid sequence comparison. Established a GMO foreign gene database which can be as a part of GMO inspection system.
The GM database in AGBIOS, which content those authorization GM crop has been to through as appraisal and release to the environment, is the origin source of those GMO foreign gene come from.
The BioPerl was used to design group operating instructions sent to NCBI GenBank to query and BLAST nucleotide sequence string interested . The free fee and stable B2D Linux Server platform was used to generated a local BLAST database which can automatically generate the associated nucleotide sequences for GMO Database. This platform also include a graphic WWWBLAST interface which can improve operational convenience and provide fast and efficient sequence alignment for GMO DNA sequencing.
