Si-Wu-tang extract stimulates bone formation through PI3K/Akt/NF-?B signaling pathways in osteoblasts

ASIA unversity > 醫學暨健康學院 > 生物科技學系 > 期刊論文 > Item 310904400/65093

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Title:	Si-Wu-tang extract stimulates bone formation through PI3K/Akt/NF-?B signaling pathways in osteoblasts
Authors:	Chi-Ming Wu;Po-Chun Chen;Te-Mao Li;Yi-Chin Fong;Chih-Hsin Tang
Contributors:	生物科技學系
Keywords:	SWT;Osteoblasts;Bone formation;Traditional Chinese medicine
Date:	2013-10
Issue Date:	2013-12-06 06:44:57 (UTC+0)
Abstract:	Background Si-Wu-Tang (SWT), a Traditional Chinese Medicine (TCM) formula, is widely used for the treatment of gynopathies diseases such as menstrual discomfort, climacteric syndrome, dysmenorrhea, and other estrogen-related diseases. Recent studies have shown that SWT can treat primary dysmenorrhea, have anti-pruritic anti-inflammatory effects, and protect against radiation-induced bone marrow damage in an animal model. It has been reported that anti-inflammatory and anti-oxidant agents have the potential to treat osteoporosis by increasing bone formation and/or suppressing bone resorption. However, the effect of SWT on bone cell function has not yet been reported. Methods Alkaline phosphatase (ALP), bone morphogenetic proteins (BMP)-2, and osteopontin (OPN) mRNA expression was analyzed by qPCR. The mechanism of action of SWT extract was investigated using western blotting. The in vivo anti-osteoporotic effect of SWT extract was assessed in ovariectomized mice. Results Here, we report that SWT increases ALP, BMP-2, and OPN expression as well as bone mineralization. In addition, we show that the PI3K, Akt, and NF-κB signaling pathways may be involved in the SWT-mediated increase in gene expression and bone mineralization. Notably, treatment of mice with SWT extract prevented bone loss induced by ovariectomy in vivo. Conclusion SWT may be used to stimulate bone formation for the treatment of osteoporosis.
Relation:	BMC Complementary and Alternative Medicine,13,277.
Appears in Collections:	[生物科技學系] 期刊論文

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